{"id":6382,"date":"2026-05-04T12:36:38","date_gmt":"2026-05-04T10:36:38","guid":{"rendered":"https:\/\/chemspx.com\/?post_type=instruments&#038;p=6382"},"modified":"2026-05-04T15:38:32","modified_gmt":"2026-05-04T13:38:32","slug":"liberty-prime-2-0-microwave-peptide-synthesizer","status":"publish","type":"instruments","link":"https:\/\/chemspx.com\/nl\/instruments\/liberty-prime-2-0-microwave-peptide-synthesizer\/","title":{"rendered":"Liberty PRIME 2.0"},"content":{"rendered":"<h2>Microwave Peptide Synthesizer<\/h2>\n<p data-start=\"52\" data-end=\"458\">The Liberty PRIME 2.0 is an<strong>\u00a0ultra-efficient microwave peptide synthesizer<\/strong>\u00a0designed for research and development. It enables fast,<strong>\u00a0high-quality peptide production<\/strong>\u00a0while significantly\u00a0<strong>reducing waste generation<\/strong>\u00a0and synthesis time. By combining CEM\u2019s patented No-Wash methodology with flexible and intuitive system controls, the system supports efficient, high-yield synthesis of structurally complex peptides.<\/p>\n<p data-start=\"460\" data-end=\"489\"><strong>Typical performance includes:<\/strong><\/p>\n<ul data-start=\"491\" data-end=\"846\">\n<li data-section-id=\"eynfqv\" data-start=\"491\" data-end=\"593\">Less than 4 minutes per amino acid addition (including coupling and deprotection, &lt;0.5 mmol scale)<\/li>\n<li data-section-id=\"q3euoq\" data-start=\"594\" data-end=\"697\">Less than 5 mL waste per amino acid addition (including coupling and deprotection, &lt;0.2 mmol scale)<\/li>\n<li data-section-id=\"11902ob\" data-start=\"698\" data-end=\"744\">Synthesis scale range from 0.005 to 5 mmol<\/li>\n<li data-section-id=\"nzlqaw\" data-start=\"745\" data-end=\"808\">High-throughput accessories for queueing up to 24 syntheses<\/li>\n<li data-section-id=\"nzlqaw\" data-start=\"745\" data-end=\"808\">cGMP peptide production compliant package available<\/li>\n<\/ul>\n<h3><strong>Unmatched Peptide Quality<\/strong><\/h3>\n<p data-start=\"907\" data-end=\"1012\">Advancements in fluidics, reaction environment, and chemistry enable improved purity and reproducibility.<\/p>\n<p data-start=\"1014\" data-end=\"1048\"><strong data-start=\"1014\" data-end=\"1048\">Chemistry innovations include:<\/strong><\/p>\n<ul data-start=\"1049\" data-end=\"1276\">\n<li data-section-id=\"gzd6s3\" data-start=\"1049\" data-end=\"1126\">Carbodiimide coupling strategy to reduce epimerization and side reactions<\/li>\n<li data-section-id=\"7izkcj\" data-start=\"1127\" data-end=\"1191\">ProTide resin development for optimized loading and swelling<\/li>\n<li data-section-id=\"1gtvuq\" data-start=\"1192\" data-end=\"1276\">Microwave-assisted reactions to enhance efficiency and overall synthesis results<\/li>\n<\/ul>\n<p data-start=\"1278\" data-end=\"1315\"><strong data-start=\"1278\" data-end=\"1315\">Engineering improvements include:<\/strong><\/p>\n<ul data-start=\"1316\" data-end=\"1522\">\n<li data-section-id=\"1r6tqd4\" data-start=\"1316\" data-end=\"1385\">Headspace gas flushing to maintain a cleaner reaction environment<\/li>\n<li data-section-id=\"e1dqax\" data-start=\"1386\" data-end=\"1441\">Optimized flow paths for efficient reagent delivery<\/li>\n<li data-section-id=\"xise1u\" data-start=\"1442\" data-end=\"1522\">Digitally variable nitrogen (N\u2082) control for bubbling, purging, and draining<\/li>\n<\/ul>\n<h3><strong>Ultra-Efficient Solid Phase Peptide Synthesis (UE-SPPS)<\/strong><\/h3>\n<p data-start=\"1577\" data-end=\"1760\">The Liberty PRIME \u2122 2.0 is built around Ultra-Efficient Solid Phase Peptide Synthesis (UE-SPPS), a methodology that eliminates traditional resin washing steps. This is achieved through:<\/p>\n<ul>\n<li>In-situ quenching of excess activated amino acids<\/li>\n<li>Controlled evaporation of excess deprotection base<\/li>\n<li>Microwave-enhanced coupling and deprotection reactions<\/li>\n<\/ul>\n<p>This approach enables high-quality peptide synthesis, including sequences up to 100 amino acids in length, while reducing total waste by up to 95% compared to conventional SPPS methods. At the same time, the system can deliver up to 95% time savings due to optimized fluidics and one-pot reaction steps.<\/p>\n<h3 data-start=\"1931\" data-end=\"2234\"><strong>Headspace gas flushing<\/strong><\/h3>\n<p data-start=\"2278\" data-end=\"2482\">A patented headspace gas flushing process removes volatile deprotection base before it can condense and re-enter the reaction mixture. This helps maintain purity, particularly in longer peptide sequences.<\/p>\n<p data-start=\"2484\" data-end=\"2505\"><strong>The process involves:<\/strong><\/p>\n<ul>\n<li>Microwave-driven deprotection reactions<\/li>\n<li>Nitrogen gas flow into the reaction vessel<\/li>\n<li>Evaporation and removal of deprotection base<\/li>\n<li>Transport of vapor and byproducts to waste<\/li>\n<li>Filtration of remaining reagents and side products<\/li>\n<\/ul>\n<h3><strong>Specialty applications<\/strong><\/h3>\n<p data-start=\"2803\" data-end=\"2971\">The Liberty PRIME 2.0 supports a wide range of synthesis scales, reagents, and applications, with automation capabilities that extend beyond standard peptide synthesis.<\/p>\n<p data-start=\"2973\" data-end=\"3015\">It can be used for automated synthesis of:<\/p>\n<ul>\n<li>Branched peptides<\/li>\n<li>Disulfide Bonding Peptoids<\/li>\n<li>High Troughput Synthesis<\/li>\n<li>Phosphopeptides<\/li>\n<li>Cyclized peptides<\/li>\n<li>PNAs<\/li>\n<li>Glycopeptides (N\/O-linked)<\/li>\n<li>N-methyl peptides<\/li>\n<li>Tagged peptides<\/li>\n<\/ul>\n<h3><strong>Liberty Blue 2.0 vs. Liberty PRIME 2.0\u00a0<\/strong><\/h3>\n<div>\n<div>\n<table>\n<tbody>\n<tr>\n<th><strong>Specification<\/strong><\/th>\n<th><a href=\"https:\/\/chemspx.com\/instruments\/liberty-blue-2-0-microwave-peptide-synthesizer\/\" target=\"_blank\" rel=\"noopener\"><strong>Liberty Blue\u202f2.0<\/strong><\/a><\/th>\n<th><strong>Liberty PRIME\u202f2.0<\/strong><\/th>\n<\/tr>\n<tr>\n<th scope=\"row\"><strong>Cycle time (at 0.1\u202fmmol)<\/strong><\/th>\n<td>&lt;\u202f4\u202fminutes per amino acid addition<\/td>\n<td>&lt;\u202f4\u202fminutes per amino acid addition<\/td>\n<\/tr>\n<tr>\n<th scope=\"row\"><strong>System waste (at 0.1\u202fmmol)<\/strong><\/th>\n<td>&lt;\u202f6\u202fmL per amino acid addition<\/td>\n<td>&lt;\u202f5\u202fmL per amino acid addition<\/td>\n<\/tr>\n<tr>\n<th scope=\"row\"><strong>Scale range<\/strong><\/th>\n<td>0.005\u20135\u202fmmol<\/td>\n<td>0.005\u20135\u202fmmol<\/td>\n<\/tr>\n<tr>\n<th scope=\"row\"><strong>Headspace flushing<\/strong><\/th>\n<td>Yes<\/td>\n<td>Yes<\/td>\n<\/tr>\n<tr>\n<th scope=\"row\"><strong>Amino acid positions<\/strong><\/th>\n<td>27<\/td>\n<td>30<\/td>\n<\/tr>\n<tr>\n<th scope=\"row\"><strong>External reagent positions<\/strong><\/th>\n<td>4 (2 activators, deprotection, wash)<\/td>\n<td>Up to 5 (2 activators, deprotection, wash, optional extra)<\/td>\n<\/tr>\n<tr>\n<th scope=\"row\"><strong>RV camera<\/strong><\/th>\n<td>Yes<\/td>\n<td>Yes<\/td>\n<\/tr>\n<tr>\n<th scope=\"row\"><strong>LED visual feedback<\/strong><\/th>\n<td>Yes<\/td>\n<td>Yes<\/td>\n<\/tr>\n<tr>\n<th scope=\"row\"><strong>High\u2011throughput options<\/strong><\/th>\n<td>HT4, HT12<\/td>\n<td>HT4, HT12, HT24<\/td>\n<\/tr>\n<tr>\n<th scope=\"row\"><strong>Optional accessories<\/strong><\/th>\n<td>N\/A<\/td>\n<td>Full cGMP compliance package<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n<p><script type=\"application\/ld+json\">\n{\n  \"@context\": \"https:\/\/schema.org\/\",\n  \"@type\": \"Product\",\n  \"name\": \"Liberty PRIME 2.0\",\n  \"image\": \"https:\/\/chemspx.com\/wp-content\/uploads\/2026\/05\/liberty-prime.webp\",\n  \"description\": \"Microwave peptide synthesizer featuring less than 4 minutes per amino acid addition and less than 5 mL waste generated per addition.\",\n  \"brand\": {\n    \"@type\": \"Brand\",\n    \"name\": \"CEM\"\n  },\n  \"offers\": {\n    \"@type\": \"Offer\",\n    \"url\": \"https:\/\/chemspx.com\/instruments\/liberty-prime-2-0-microwave-peptide-synthesizer\/\",\n    \"priceCurrency\": \"EUR\",\n    \"price\": \"0.00\",\n    \"availability\": \"https:\/\/schema.org\/InStock\",\n    \"seller\": {\n      \"@type\": \"Organization\",\n      \"name\": \"ChemSPX\",\n      \"url\": \"https:\/\/chemspx.com\",\n      \"logo\": \"https:\/\/chemspx.com\/wp-content\/uploads\/2025\/01\/ChemSPX-logo.png\",\n      \"areaServed\": [\n        { \"@type\": \"Country\", \"name\": \"Belgium\" },\n        { \"@type\": \"Country\", \"name\": \"Netherlands\" },\n        { \"@type\": \"Country\", \"name\": \"Luxembourg\" }\n      ]\n    }\n  }\n}\n<\/script><\/p>\n<p><script type=\"application\/ld+json\">\n{\n  \"@context\": \"https:\/\/schema.org\",\n  \"@type\": \"FAQPage\",\n  \"mainEntity\": [{\n    \"@type\": \"Question\",\n    \"name\": \"How does the Liberty PRIME\u2122 2.0 differ from conventional peptide synthesizers?\",\n    \"acceptedAnswer\": {\n      \"@type\": \"Answer\",\n      \"text\": \"The system combines CEM\u2019s patented No\u2011Wash methodology, microwave\u2011assisted chemistry, and advanced fluidics. 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Vapors and byproducts are transported to waste while remaining reagents are filtered, preventing contamination of the growing peptide chain.\"\n    }\n  },{\n    \"@type\": \"Question\",\n    \"name\": \"How much time savings can be achieved compared to conventional SPPS?\",\n    \"acceptedAnswer\": {\n      \"@type\": \"Answer\",\n      \"text\": \"Due to optimized fluidics, elimination of wash steps, and one\u2011pot microwave reactions, the Liberty PRIME\u2122 2.0 can deliver up to 95% time savings compared with traditional peptide synthesis workflows.\"\n    }\n  }]\n}\n<\/script><\/p>\n","protected":false},"excerpt":{"rendered":"<p> Ultra-efficient microwave peptide synthesizer that delivers high-quality peptides with record low waste generation and synthesis 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