{"id":6386,"date":"2026-05-04T13:50:56","date_gmt":"2026-05-04T11:50:56","guid":{"rendered":"https:\/\/chemspx.com\/?post_type=instruments&p=6386"},"modified":"2026-05-04T15:37:50","modified_gmt":"2026-05-04T13:37:50","slug":"liberty-lite-2-0-microwave-peptide-synthesizer","status":"publish","type":"instruments","link":"https:\/\/chemspx.com\/nl\/instruments\/liberty-lite-2-0-microwave-peptide-synthesizer\/","title":{"rendered":"Liberty Lite 2.0"},"content":{"rendered":"

Microwave Peptide Synthesizer<\/h2>\n

The Liberty Lite\u2122 2.0 is an entry-level microwave peptide synthesizer<\/strong> within the Liberty 2.0 series, that delivers efficient and automated solid phase peptide synthesis<\/strong> (SPPS). By applying patented microwave irradiation during both deprotection and coupling steps, the system enables faster reaction cycles and controlled synthesis conditions.<\/p>\n

With typical cycle times of approximately 12 minutes<\/strong> (at 0.1 mmol scale), the Liberty Lite 2.0 supports rapid peptide production while maintaining efficient solvent usage and controlled waste generation.<\/p>\n

Performance and system design<\/strong><\/h3>\n

The system combines microwave technology with precise process control to improve synthesis efficiency and reproducibility. In-situ temperature monitoring is achieved through an internal fiber-optic probe, providing accurate and responsive temperature control throughout the reaction.<\/p>\n

The patented Flex-Add\u2122 reagent delivery system ensures controlled addition of critical reagents, supporting consistent reaction conditions.<\/p>\n

Key system specifications include:<\/strong><\/p>\n

    \n
  • 12-minute cycle times for coupling and deprotection steps<\/li>\n
  • Synthesis scale range from 0.005 to 5 mmol<\/li>\n
  • 27 amino acid positions and 2 activation reagent positions<\/li>\n
  • Flexible scale handling using three reaction vessel sizes<\/li>\n<\/ul>\n

    Automation\u00a0<\/strong><\/h3>\n

    The Liberty Lite 2.0 enables straightforward programming and operation. As a fully automated microwave SPPS system, it simplifies peptide synthesis workflows while maintaining flexibility in chemistry.<\/p>\n

    For increased throughput, you can upgrade the system with the HT4 module, enabling automated queueing of up to four peptide syntheses in a single run.<\/p>\n

    Microwave control and temperature accuracy<\/strong><\/h3>\n

    Microwave irradiation is used as part of the patented coupling and deprotection methodology, allowing efficient energy transfer and improved reaction control. Temperature is continuously monitored via an internal fiber-optic sensor, ensuring accurate measurement directly within the reaction environment.<\/p>\n

    Applications<\/strong><\/h3>\n

    The Liberty Lite\u2122 2.0 supports the synthesis of both standard and structurally complex peptides. Like other systems in the Liberty 2.0 series, it utilizes High-Efficiency Solid Phase Peptide Synthesis (HE-SPPS) and CarboMAX coupling methodologies.<\/p>\n

    Supported applications include:<\/strong><\/p>\n

      \n
    • Branched peptides<\/li>\n
    • Cyclized peptides<\/li>\n
    • Disulfide bond formation<\/li>\n
    • Glycopeptides (N- and O-linked)<\/li>\n
    • Phosphopeptides<\/li>\n
    • N-methyl peptides<\/li>\n
    • Peptoids<\/li>\n
    • High-throughput peptide synthesis<\/li>\n<\/ul>\n

      Liberty Lite 2.0 vs. Liberty Blue 2.0 vs. Liberty PRIME 2.0\u00a0<\/strong><\/h3>\n
      \n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
      Specification<\/strong><\/th>\nLiberty Lite\u202f2.0<\/strong><\/th>\nLiberty Blue\u202f 2.0<\/strong><\/a><\/th>\nLiberty PRIME 2.0<\/strong><\/a><\/th>\n<\/tr>\n
      Cycle time (at 0.1\u202fmmol)<\/strong><\/th>\n12\u202fminutes<\/td>\n4\u202fminutes<\/td>\n2.5\u202fminutes<\/td>\n<\/tr>\n
      System waste (at 0.1\u202fmmol)<\/strong><\/th>\n32\u202fmL<\/td>\n16\u202fmL<\/td>\n10\u202fmL<\/td>\n<\/tr>\n
      Scale range<\/strong><\/th>\n0.005\u20135\u202fmmol<\/td>\n0.005\u20135\u202fmmol<\/td>\n0.005\u20135\u202fmmol<\/td>\n<\/tr>\n
      Ultra\u2011purity headspace flushing<\/strong><\/th>\nNo<\/td>\nYes<\/td>\nYes<\/td>\n<\/tr>\n
      NO WASH (UE\u2011SPPS)<\/strong><\/th>\nNo<\/td>\nYes<\/td>\nYes<\/td>\n<\/tr>\n
      Amino acid positions<\/strong><\/th>\n27<\/td>\n27<\/td>\n30<\/td>\n<\/tr>\n
      External reagent positions<\/strong><\/th>\n4<\/td>\n4<\/td>\n5<\/td>\n<\/tr>\n
      RV camera<\/strong><\/th>\nNo<\/td>\nYes<\/td>\nYes<\/td>\n<\/tr>\n
      LED visual feedback<\/strong><\/th>\nNo<\/td>\nYes<\/td>\nYes<\/td>\n<\/tr>\n
      Multiple peptide synthesis options<\/strong><\/th>\nUp to 4 (HT4)<\/td>\nUp to 4 (HT4)
      \nUp to 12 (HT12)<\/td>\n      		Up to 4 (HT4)
      \nUp to 12 (HT12)
      \nUp to 24 (HT24)<\/td>\n<\/tr>\n
      Optional accessories<\/strong><\/th>\nN\/A<\/td>\nN\/A<\/td>\nFull cGMP compliance package<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n