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Emulsion Breaker
Fluo-Break is a clear fluorinated emulsion breaker that efficiently disrupts microfluidic droplets and enables recovery of the aqueous phase. It works with emulsions stabilized using Emulseo surfactants and fluorinated oils and supports droplet systems based on these materials.
The product is intended for workflows where controlled droplet breaking is required, including applications such as cell recovery and downstream analysis in microfluidic systems. It specifically works with Emulseo’s surfactants and oils (Surfactants for droplet stabilisation and Fluorinated oils), allowing easy recovery of the droplet phase.
How to use Fluo-Break?
- Transfer the emulsion into a microcentrifuge tube. The upper phase is the emulsion and the lower phase is the excess of FluoSurf diluted in Fluo-Oil.
- Carefully remove the excess oil (bottom phase) using a pipette tip.
- Add Fluo-Break to the emulsion (approximately 1× emulsion volume).
- Close the tube and shake it manually. The emulsion will begin to break.
- Centrifuge the tube for 1 minute until complete phase separation occurs. The upper phase is the aqueous phase and the lower phase is a mixture of Fluo-Break and fluorinated oil.
- Remove the upper (aqueous) phase using a pipette tip and transfer it to a clean microcentrifuge tube. You can then use the aqueous phase for further experiments.
Applications
- Diagnostics: digital droplet PCR (ddPCR) for DNA analysis
- Biotechnology: single-cell analysis for stem cell research and immune analysis
- Pharmaceutical development: screening of potential drugs
- Encapsulation: stabilisation of dyes or small molecules in droplets
Benefits
- Gebruiksgemak
- Efficient recovery of the droplet phase
- Compatible with a range of surfactant and oil systems
- Compatible with Emulseo surfactants and fluorinated oils
Storage and handling
You can store Fluo-Break at room temperature in its original vial for up to six months without performance loss. Standard laboratory safety precautions apply, including protective clothing and eye protection, and users should refer to the relevant safety data sheet before use.
