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Microwave Peptide Synthesizer
The Liberty Liteâ„¢ 2.0 is an entry-level microwave peptide synthesizer within the Liberty 2.0 series, that delivers efficient and automated solid phase peptide synthesis (SPPS). By applying patented microwave irradiation during both deprotection and coupling steps, the system enables faster reaction cycles and controlled synthesis conditions.
With typical cycle times of approximately 12 minutes (at 0.1 mmol scale), the Liberty Lite 2.0 supports rapid peptide production while maintaining efficient solvent usage and controlled waste generation.
Performance and system design
The system combines microwave technology with precise process control to improve synthesis efficiency and reproducibility. In-situ temperature monitoring is achieved through an internal fiber-optic probe, providing accurate and responsive temperature control throughout the reaction.
The patented Flex-Addâ„¢ reagent delivery system ensures controlled addition of critical reagents, supporting consistent reaction conditions.
Key system specifications include:
- 12-minute cycle times for coupling and deprotection steps
- Synthesis scale range from 0.005 to 5 mmol
- 27 amino acid positions and 2 activation reagent positions
- Flexible scale handling using three reaction vessel sizes
AutomatiseringÂ
The Liberty Lite 2.0 enables straightforward programming and operation. As a fully automated microwave SPPS system, it simplifies peptide synthesis workflows while maintaining flexibility in chemistry.
For increased throughput, you can upgrade the system with the HT4 module, enabling automated queueing of up to four peptide syntheses in a single run.
Microwave control and temperature accuracy
Microwave irradiation is used as part of the patented coupling and deprotection methodology, allowing efficient energy transfer and improved reaction control. Temperature is continuously monitored via an internal fiber-optic sensor, ensuring accurate measurement directly within the reaction environment.
Applications
The Liberty Liteâ„¢ 2.0 supports the synthesis of both standard and structurally complex peptides. Like other systems in the Liberty 2.0 series, it utilizes High-Efficiency Solid Phase Peptide Synthesis (HE-SPPS) and CarboMAX coupling methodologies.
Supported applications include:
- Branched peptides
- Cyclized peptides
- Disulfide bond formation
- Glycopeptides (N- and O-linked)
- Phosphopeptides
- N-methyl peptides
- Peptoids
- High-throughput peptide synthesis
Liberty Lite 2.0 vs. Liberty Blue 2.0 vs. Liberty PRIME 2.0Â
| Specification | Liberty Lite 2.0 | Liberty Blue  2.0 | Liberty PRIME 2.0 |
|---|---|---|---|
| Cycle time (at 0.1 mmol) | 12 minutes | 4 minutes | 2.5 minutes |
| System waste (at 0.1 mmol) | 32 mL | 16 mL | 10 mL |
| Scale range | 0.005–5 mmol | 0.005–5 mmol | 0.005–5 mmol |
| Ultra‑purity headspace flushing | No | Yes | Yes |
| NO WASH (UE‑SPPS) | No | Yes | Yes |
| Amino acid positions | 27 | 27 | 30 |
| External reagent positions | 4 | 4 | 5 |
| RV camera | No | Yes | Yes |
| LED visual feedback | No | Yes | Yes |
| Multiple peptide synthesis options | Up to 4 (HT4) | Up to 4 (HT4) Up to 12 (HT12) |
Up to 4 (HT4) Up to 12 (HT12) Up to 24 (HT24) |
| Optional accessories | N/A | N/A | Full cGMP compliance package |
