The TFF system enables a fast and efficient process for separation and purification of biomolecules. Fluid filtration can be either microfiltration or ultrafiltration, depending on the membranes used, and is performed in parallel to the membrane and through a pressure difference, allowing smaller volume components to pass through the filter while larger volume ones circulate along the membrane, preventing membrane fouling. This allows for continuous flow and increased yield.
The product that passes through the filter is called permeate, while the one that continues its trajectory is called retentate, which is recycled back into the feed. The TFF equipment consists of two main modules. The first one is the filtration assembly, which includes a single-use plastic tank with a volume of 2 to 5 liters that contains the product to be filtered and a filtration membrane with a surface up to 0.7 m².
The bag is supported on a support where it is located by a load cell and has a conical bottom to prevent product loss. The plastic tank is fixed on a support and contains the tangential filter that purifies the biomolecules. Both the filter and the support are gamma irradiated beforehand.