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Microwave Peptide Synthesizer
The Liberty Blue™ 2.0 is an ultra-efficient microwave peptide synthesizer designed for research and development. It enables fast, high-quality peptide production while significantly reducing waste generation and synthesis time. By combining CEM’s patented No-Wash methodology with flexible and intuitive system controls, the system supports efficient, high-yield synthesis of structurally complex peptides.
Typical performance includes:
- Less than 4 minutes per amino acid addition (including coupling and deprotection, <0.5 mmol scale)
- Less than 6 mL waste per amino acid addition (including coupling and deprotection, <0.2 mmol scale)
- Synthesis scale range from 0.005 to 5 mmol
- High-throughput accessories for queueing up to 12 syntheses
- 21 CFR Part 11 compliant software
How does the Liberty Blue 2.0 improve peptide quality?
Advancements in fluidics, reaction environment, and chemistry enable improved purity and reproducibility.
Chemistry innovations include:
- Carbodiimide coupling strategy to reduce epimerization and side reactions
- ProTide resin development for optimized loading and swelling
- Microwave-assisted reactions to enhance efficiency and overall synthesis results
Engineering improvements include:
- Headspace gas flushing to maintain a cleaner reaction environment
- Optimized flow paths for efficient reagent delivery
- Digitally variable nitrogen (N₂) control for bubbling, purging, and draining
Ultra-Efficient Solid Phase Peptide Synthesis (UE-SPPS)
The Liberty Blue™ 2.0 is built around Ultra-Efficient Solid Phase Peptide Synthesis (UE-SPPS), a methodology that eliminates traditional resin washing steps. This is achieved through:
- In-situ quenching of excess activated amino acids
- Controlled evaporation of excess deprotection base
- Microwave-enhanced coupling and deprotection reactions
This approach enables high-quality peptide synthesis, including sequences up to 100 amino acids in length, while reducing total waste by up to 95% compared to conventional SPPS methods. At the same time, the system can deliver up to 95% time savings due to optimized fluidics and one-pot reaction steps.
Headspace gas flushing
A patented headspace gas flushing process removes volatile deprotection base before it can condense and re-enter the reaction mixture. This helps maintain purity, particularly in longer peptide sequences.
The process involves:
- Microwave-driven deprotection reactions
- Nitrogen gas flow into the reaction vessel
- Evaporation and removal of deprotection base
- Transport of vapor and byproducts to waste
- Filtration of remaining reagents and side products
Specialty applications
The Liberty Blue™ 2.0 supports a wide range of synthesis scales, reagents, and applications, with automation capabilities that extend beyond standard peptide synthesis.
It can be used for automated synthesis of:
- Branched peptides
- Disulfide Bonding Peptoids
- High Throughput Synthesis
- Phosphopeptides
- Cyclized Peptides
- PNAs
- Glycopeptides (N/O-linked)
- N-methyl peptides
- Stapled peptides
- Proteins
Liberty Blue 2.0 vs. Liberty PRIME 2.0
| Specification | Liberty Blue 2.0 | Liberty PRIME 2.0 |
|---|---|---|
| Cycle time (at 0.1 mmol) | < 4 minutes per amino acid addition | < 4 minutes per amino acid addition |
| System waste (at 0.1 mmol) | < 6 mL per amino acid addition | < 5 mL per amino acid addition |
| Scale range | 0.005–5 mmol | 0.005–5 mmol |
| Headspace flushing | Yes | Yes |
| Amino acid positions | 27 | 30 |
| External reagent positions | 4 (2 activators, deprotection, wash) | Up to 5 (2 activators, deprotection, wash, optional extra) |
| RV camera | Yes | Yes |
| LED visual feedback | Yes | Yes |
| High‑throughput options | HT4, HT12 | HT4, HT12, HT24 |
| Optional accessories | N/A | Full cGMP compliance package |
