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Automated Parallel Peptide Synthesizer
The MultiPep 1 is a flexible and cost-efficient automated parallel peptide synthesizer designed for high-throughput peptide screening. It supports multiple synthesis formats in parallel, including plates, columns, and cellulose membrane-based approaches, enabling the production of large peptide libraries.
Flexible synthesis formats
The system accommodates different experimental scales and formats:
- Plates: up to 96 peptides at 1–10 μmol scale
- Columns: up to 48 peptides at 1–10 μmol, or 8 peptides at 10–300 μmol
- SPOT synthesis: up to 1200 peptides on two cellulose membranes
- CelluSpots™ format: up to 768 peptides on dissolvable cellulose support
This flexibility allows users to adapt the system to both small-scale screening and larger parallel synthesis workflows.
Elevated temperature synthesis
An integrated heating option enables elevated temperature peptide synthesis in both 96-well plate and column formats. Adjustable heating accessories allow controlled temperature conditions, which can improve synthesis quality, particularly for difficult or longer peptide sequences.
Key system features
The MultiPep 1 includes several operational features to support efficient parallel synthesis:
- Fast parallel synthesis with an 8-position washing arm
- Vortex mixing for improved reaction homogeneity
- Pre-activation or in-situ activation chemistry options
- Heating module for temperature-controlled synthesis
Peptide library production
The system is designed for efficient peptide library generation using both plate and column formats. It supports:
- High-density libraries using 96-well plates (up to 96 peptides in parallel)
- Mini-column synthesis for up to 48 peptides in parallel
- Larger column formats (2, 5, or 10 mL) for up to 8 parallel syntheses
This enables flexible scaling depending on peptide complexity and throughput requirements.
SPOT synthesis for high-throughput screening
SPOT synthesis enables the production of immobilized peptide microarrays on cellulose membranes. With the MultiPep 1, up to 1200 peptides can be synthesized per batch. These arrays are used for:
- Binding studies
- Cell-based assays
- Solution-phase screening applications
CelluSpots technology
CelluSpots technology enables the creation of multiple identical peptide array copies on glass slides. It combines SPOT synthesis with a dissolvable cellulose support and is used together with a slide spotting process to replicate arrays for downstream applications.
Peptide nucleic acid (PNA) synthesis
The system also supports small-scale peptide nucleic acid synthesis, which is useful for expensive monomers. It allows synthesis at scales as low as 1 μmol and can run up to 48 parallel reactions using small-volume columns (250 μL or 500 μL formats).
Software and control
The MultiPep 1 includes software designed for intuitive operation and flexible experiment setup. Core functions include:
- Import and export of peptide sequences
- Preconfigured synthesis protocols
- Reagent consumption calculations
- Real-time monitoring of synthesis runs
- Full documentation of each experiment
MultiPep 1 vs. MultiPep 2
| Feature | MultiPep 1 | MultiPep 2 |
|---|---|---|
| Scale range | 0.001 – 0.3 mmol | 0.001 – 0.5 mmol |
| Synthesis formats – Well plates | 1 × 96‑well plate | 4 × 96‑well plates |
| Synthesis formats – Mini‑columns | 24 / 48 mini‑columns (250, 500 µL) | 24 / 48 mini‑columns (250, 500 µL) |
| Synthesis formats – Columns | 8 columns (2, 5, 10 mL) | 48 columns (2, 5, 10, 20 mL) 72 columns (2, 5, 10 mL) |
| Micro peptide arrays | SPOT synthesis on cellulose membranes Up to 1,200 peptides in parallel on 2 sheets |
SPOT synthesis on cellulose membranes Up to 2,400 peptides in parallel on 4 sheets |
| Copies of arrays (CelluSpots) | Many copies of arrays up to 768 peptides each using SlideSpotter | Many copies of arrays up to 768 peptides each using SlideSpotter |
| Amino acid positions | 26 standard (up to 48) | 31 standard (up to 48) |
| Other positions | Up to 15 | Up to 20 |
| Fluid delivery | Digital syringe pump | Digital syringe pump |
| Dimensions (W × D × H) | 22.8″ × 20.9″ × 28.3″ (58 × 53 × 72 cm) |
35.0″ × 25.6″ × 31.1″ (89 × 65 × 79 cm) |
| Accessories | CleavagePro™ SlideSpotter — CelluSpots |
CleavagePro™ SlideSpotter — CelluSpots |
